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  • the role of endothelial cells in the retinal stem and progenitor cell niche within a 3d engineered hydrogel matrix

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1391/01/01
    • تاریخ انتشار در تی پی بین: 1391/01/01
    • تعداد بازدید: 718
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    • شماره تماس دبیرخانه رویداد: -
     cell–cell interactions are critical to understanding functional tissues. a number of stem cell populations have been shown to receive key regulatory information from endothelial cells (ecs); however, the role of ecs in the retinal stem and progenitor cell (rspc) niche has been largely unexplored. to gain greater insight into the role of ecs on rspc fate, a three-dimensional (3d) co-culture model, incorporating cell–cell interactions, was designed by covalently-modifying agarose hydrogels with growth factors and cell-adhesive peptides in defined volumes. therein ecs adopted tubular-like morphologies similar to those observed in vivo, but not observed in two-dimensional (2d) cultures. unexpectedly, ecs inhibited proliferation and differentiation of rspcs, revealing, for the first time, the possible role of ecs on rspc fate. this 3d hydrogel scaffold provides a simple, reproducible and versatile method with which to answer biological questions related to the cellular microenvironment.

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