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  • comparison of three screening methods to select mixed-microbial inoculum for mixed-acid fermentations

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 668
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     using a mixed culture of microorganisms, the carboxylate platform converts biomass into hydrocarbons and chemicals. to develop a method that identifies the highest performing inoculum for carboxylate fermentations, five bacterial communities were screened and ranked by three fermentation performance tests: (1) 30-day batch screen, (2) 28-day continuum particle distribution model (cpdm), and (3) 5-month continuous countercurrent fermentation trains. to screen numerous inocula sources, these tests were used sequentially in an aseptic environment. for the batch-fermentation screen, inoculum 1 achieved the highest conversion. for the cpdm evaluation, the operating map for inoculum 1 had the highest performance. for the continuous countercurrent fermentation, the train resulting from inoculum 1 was among the best performers. this study suggests that the three screens are a useful and predictive method for choosing optimal inocula sources. the bacterial community with optimal performance in these three screens could be considered for use in commercial-scale fermentations.

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