wound healing effect of hydroalcoholic extract of salvadora persica (miswak) on physicaly induced second-degree burn wound in balb/c mice

wound healing effect of hydroalcoholic extract of salvadora persica (miswak) on physicaly induced second-degree burn wound in balb/c mice

introduction: different scholars have mentioned a lot of benefits for salvadora persica(sp) compounds. the present study was conducted aiming at evaluating the effect of hydroalcoholic extract of sp on second-degree burn healing in mice.materials and methods: the present study population was 60 mature mice (balb/c). the mice were divided into 5 equal groups.

groups 1 and 2 were treated by means of the application of 5% hydroalcoholic extract of sp and 10% extract of it, respectively; regarding group 3 revieved silver sulfadiazine ointment. group 4 was treated using vaseline. group 5 received no treatment. then, samples from the injured sites was gathered, and wound healing was evaluated histopathologically.

results: inflammation and infilteration of nutrophils and lymphocytes in the treatment groups, i.e. the groups to which 5% and 10% sp extract was applied, demonstrated a significant decrease, compared to the vaseline and sham groups (p<0.01). moreover, the number of fibroblasts increased followed by collagen production, epithelialization and new hair follicles formation at the wound margins on the 10th and 14th day in the 5% and 10% sp extract groups significantly increased compared to vaseline and sham groups (p<0.05).

the number of fibroblasts and the density of collagen tissues in the group treated with 10% sp extract even showed a significant increase compared to the groups treated with 5% sp extract and silver sulfadiazine on the 14th day.conclusion: it was found that hydroalcoholic salvadora persica extract accelerated the healing of second-degree burn injuries in balb/c mice.