• detection of high risk human papillomaviruses in esophageal squamous cell carcinoma by pcr and dna sequencing

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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
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     background and objectives: human papillomavirus (hpv) is one of the possible etiologic factors in development of esophageal squamous cell carcinoma (escc) especially in high risk areas. we aimed to study the frequency of high risk hpvs in escc. material and methods: 140 cases of escc were analyzed for the hpv dna by polymerase chain reaction (pcr) using gp5+/gp6+ primers to amplify a 150-bp segment of hpv genome. hpv positive samples were subsequently sequenced to identify the type of hpv. results: from 140 patients with escc enrolled to our study, 50.7% were female and 49.3% were male, aged between 20 to 81 years old. half of them were between 60-70 years. 23.6% of tumoral regions and 8.6% of non-involved tumor margins were hpv positive. the hpv positive cases were 21.7% male and 25.3 % female. from hpv positive tumor cases 36% were also positive in noninvolved tumor margins. the frequency of hpv subtypes in tumoral regions were as follow: hpv-16:60.6%, hpv-18: 30.3%, hpv-33: 6.1% and hpv-31: 3 %. we found only hpv-16 in tumor margins. there is no correlation between presence and types of hpv with patients' sex and age. conclusion: our results confirm the previously reported hpv involvement in the esophageal squamous cell carcinoma in high-risk areas. hpv-16 and 18 were the most prevalent types of hpv among the esophageal cancer cases.

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