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  • extractive fermentation for improved production and recovery of lipase derived from burkholderia cepacia using a thermoseparating polymer in aqueous two-phase systems

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 650
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -

    an extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular burkholderia cepacia lipase. a 10% (w/w) solution of ethylene oxide–propylene oxide (eopo) with a molecular mass of 3900 g/mol and ph 8.5, a 200 rpm speed, and 30 °c were selected as the optimal conditions for lipase production (55 u/ml). repetitive batch fermentation was performed by continuous replacement of the top phase every 24 h, which resulted in an average cell growth mass of 4.7 g/l for 10 extractive batches over 240 h. in scaling-up the process, a bench-scale bioreactor was tested under the conditions that had been optimized in flasks. the production rate and recovery yield were higher in the bioreactor compared to fermentation performed in flasks.

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