• adhesion to fibronectin induces p27kip1 nuclear accumulation through down-regulation of jab1 and contributes to cell adhesion-mediated drug resistance (cam-dr) in rpmi 8,226 cells

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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
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     mounting evidence has been shown that integrin-mediated cellular adhesion confers resistance to chemotherapy of multiple myeloma. the molecular mechanism underlying cell adhesion-mediated drug resistance (cam-dr) is, however, poorly understood. in this report, we demonstrated that rpmi 8,226 cells accumulated p27kip1 in the nucleus when they were adhered to fibronectin (fn). the adhesion-mediated p27kip1 nuclear recruitment was regulated via the down-regulation of jab1, a negative regulator of cell cycle. overexpression of jab1 reversed the elevated p27kip1 in the nucleus, which needed phosphorylation of p27kip1 on serine 10, whereas inhibition of jab1 by sirna further increased the elevated p27kip1. furthermore, we found overexpression of jab1 did not affect 8,226 cells adhesion to fn, but reversed doxorubicin or mitoxantrone-induced cam-dr phenotype. in conclusion, our data suggest that jab1 plays an important role in cam-dr, which depends on pser10-p27kip1-mediated subcellular localization of p27kip1. the understanding of this novel molecular mechanism may prove valuable in designing new therapeutic approaches for cam-dr in multiple myeloma.

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