the effects of biogenic bismuth oxide nanoparticles on radiosensitivity of gastric cancer cells

the effects of biogenic bismuth oxide nanoparticles on radiosensitivity of gastric cancer cells

gastric cancer is the 5th most common neoplasm and the 3rd most deadly cancer worldwide. in this study, we produced bismuth oxide (bi2o3) and bismuth oxide-zeolite nanocomposites (bi2o3-z ncs) using vibrio sp. vlc bacteria and investigated their effects on radiosensitivity of human gastric cancer cells. after synthesis of bi2o3 nps and bi2o3-z ncs by vibrio sp.

vlc bacteria, the characterization of nps were evaluated using uv-visible, ftir, xrd, dls, zeta potential, tem and fesem. then, mkn-45 cells, a human gastric adenocarcinoma cell line, were pretreated with 25 μg/ml bi2o3 nps (in two forms of heated and non-heated) and bi2o3-z ncs, while bismuth salt and zeolite were considered as controls. after 24 h, cells were exposed to 200, 400 and 600 centigray (cgy) of x-radiation and recovered for 72 h. at the end, viability of cells was determined by resazurin assay.

findings of present research indicated that bi2o3 nps (non-heated) as well as bismuth salt and zeolite pretreatments increased the effects of 200 cgy radiation. moreover, bi2o3 nps (heated) significantly (p <0.05) improved the sensitivity of mkn-45 cells to 400 cgy x-ray. more interesting results were observed when 600 cgy radiation was applied, as bi2o3 nps (heated) and bi2o3-z ncs significantly (p <0.0001 and p <0.05, respectively) enhanced radiosensitivity of cells. in conclusion, obtained results revealed that bi2o3 nps and bi2o3-z ncs could act as potent radio sensitizers, although more investigation on other gastric cell lines is required to confirm our findings.